A Siriraj team validated the Mindray BS-800 hemolysis index for plasma free hemoglobin against the Harboe method, reporting high precision and correlation across the evaluated range and a 2 mg/dL detection limit. High bilirubin caused up to 15% negative bias, and analytical validation does not establish clinical-outcome benefit.
Key findings
- Within-run precision was 0.41-1.89% and between-run precision 0.71-2.10%; linearity spanned 2.3-507.9 mg/dL. Detection and quantitation limits were 2 and 5 mg/dL. Triglyceride up to 520.9 mg/dL caused no notable interference, but bilirubin at 14.2-34.7 mg/dL caused up to 15% negative bias. At 0-130 mg/dL, r=0.9967 and HI=1.0886×Harboe-0.3071.
Why this matters globally
After local verification, this method could speed and simplify hemolysis monitoring in hematology and transfusion services using this analyzer. Performance should not be assumed for other analyzer models.
Thai researcher contribution
The Faculty of Medicine Siriraj Hospital, Mahidol University performed a multidimensional validation connecting hematology needs with laboratory quality practice.
Limitations to consider
The abstract omits sample counts and reference-population details. High correlation does not establish agreement across all concentrations, and bilirubin is an important interference. Diluted EDTA plasma may differ from routine workflow, and a single-analyzer, single-center validation requires local lot, calibration, and specimen verification.
Verify the original sources
Journal of Clinical Laboratory AnalysisRead the original article↗DOI: 10.1002/jcla.70303