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Evidence of global relevance

Metabolic disruption associated with e-cigarette vaping and secondhand exposure: Urinary metabolomics evidence from a Thai population

A Thai urinary-metabolomics study compared active e-cigarette users, people exposed to secondhand aerosol, and unexposed controls. Direct use was associated with amino-acid, mitochondrial-energy, and glutathione pathways, while secondhand exposure was linked mainly to alanine-aspartate-glutamate metabolism. These are biological-response associations, not proof that exposure caused disease.

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Key findings

  • Direct use was associated with amino-acid, energy, and redox pathways; glutathione metabolism was prominent and oxidised glutathione was a candidate biomarker. Secondhand exposure was associated with alanine, aspartate, and glutamate metabolism, with L-glutamic acid showing discriminatory potential. Propylene glycol, glycerol, cotinine N-oxide, and nicotine-related metabolites were candidate exposure markers.
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Why this matters globally

Systemic evidence on secondhand e-cigarette aerosol remains limited. Metabolomics may distinguish exposure markers from biological-effect markers, informing surveillance and clean-air policy, but it is not ready for individual diagnosis.

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Thai researcher contribution

Chiang Mai University, Chulalongkorn University, Payap University, and Chao Phraya Abhaibhubejhr Hospital generated Thai population evidence. Publisher metadata corrects a secondary-database mismatch involving the hospital name.

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Limitations to consider

The abstract does not report sample size, age, device type, dose, cotinine levels, or control for diet, medicines, combustible smoke, and comorbidity. A comparative design cannot establish temporality, and multivariate metabolomics is vulnerable to overfitting and multiple testing.

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Verify the original sources

Toxicology ReportsRead the original article

DOI: 10.1016/j.toxrep.2026.102313

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