Researchers developed a TaqMan real-time PCR assay targeting psittacine CHD markers and tested 1,087 samples across 38 species and 34 genera. Results were fully concordant with conventional PCR, with reported sensitivity down to 0.031 ng DNA.
Key findings
- The assay achieved 100% agreement with conventional PCR in the evaluated set and detected as little as 0.031 ng DNA. Real-time detection reduces post-amplification handling, while psittacine-specific markers may support combined species-and-sex forensic workflows.
Why this matters globally
Reliable sex determination can improve breeding management, reduce unnecessary handling and support scrutiny of trade claims. With independent validation, the assay could be transferred to conservation and forensic laboratories internationally.
Thai researcher contribution
Key Thai researchers from Prince of Songkla University developed and validated the method with an international collaborator, demonstrating Thai capacity in wildlife forensic genetics.
Limitations to consider
The 100% figure is agreement with conventional PCR, not an independent gold-standard sex determination for every specimen. Untested species and highly degraded samples may behave differently, and laboratory accuracy does not itself demonstrate reduced illegal trade.