ANTIOXIDANT AND ANTI-APOPTOTIC ACTIVITIES OF MAKIANG FRUIT EXTRACT IN ARPE-19 CELLS EXPOSED TO GLUCOSE-INDUCED CELLULAR DAMAGE

Information from the abstract

Diabetic retinopathy (DR) is a leading cause of visual loss resulting from microvascular complications of diabetes mellitus. In DR, retinal pigment epithelial (RPE) cells are exposed to elevated blood sugar levels for prolonged periods, leading to oxidative stress and apoptosis. Makiang fruit (Cleistocalyx nervosum var. paniala) is rich in anthocyanins (ACN), which possess antioxidant and anti-apoptotic properties. However, its protective effects on RPE cells against glucose-induced oxidative stress have not been studied. This study aimed to examine the effects of Makiang fruit extract on human retinal pigment epithelial (ARPE-19) cells exposed to glucose-induced oxidative stress. Makiang fruits were extracted with 95% ethanol (MKE95). The results showed that MKE95 exhibited antioxidant capacity as measured by the DPPH, ORAC, and FRAP assays. For the cell-based experiments, ARPE-19 cells were co-treated with MKE95 (50–200 μg/mL) and 0.5% DMSO in DMEM/F-12 (5.5 mM glucose), with D-glucose adjusted to 30 mM. At the highest concentration, MKE95 significantly (p < 0.05) improved cell viability by 15.4%, reduced intracellular ROS by 37.8%, and decreased caspase-9 and -3 activities (1.66- and 1.64-fold, respectively) compared to the glucose-treated group. It also enhanced antioxidant defenses, increasing SOD, CAT, GPx activities, and GSH levels by 22.4%, 26.3%, 24.7%, and 24.4%, respectively, in a dose-dependent manner. However, these findings are derived from an in vitro model. Although Makiang extract demonstrates protective effects against glucose-induced oxidative stress in RPE cells, further in vivo and clinical studies are necessary to confirm its potential role in preventing diabetic retinopathy.

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Related topics: Retinal Diseases and Treatments · Natural Antidiabetic Agents Studies · Retinal Imaging and Analysis