Effective Butanol Production from Sugarcane Molasses by Immobilized Clostridium beijerinckii in Batch and Fed-Batch Fermentations Integrated with Product Recovery

Information from the abstract

This study investigated the enhancement of an acetone–butanol–ethanol (ABE) fermentation from sugarcane molasses using Clostridium beijerinckii TISTR 1461 immobilized on lotus stalk (LS) pieces. The addition of 0.01 g/L of ZnSO4, MnSO4 or FeSO4 into the molasses medium containing 50 g/L of sugar negatively impacted butanol production. However, incorporating 2.2 g/L of ammonium acetate as a buffer increased the butanol concentration (PB), ABE concentration (PABE), and butanol productivity (QB) by 8–11%. Optimization of the initial sugar concentration in batch mode showed that 80 g/L yielded the highest PB (19.65 g/L) and QB (0.55 g/L·h). To further improve the butanol production efficiency, two strategies were employed: fed-batch process and gas stripping (GS) for product recovery. Integrating a GS system into the batch process increased the PB (22.26 g/L), and QB (0.61 g/L·h) by ~11–13%. In a fed-batch mode (an initial sugar concentration of 50 g/L at 50% of the total working volume), feeding a medium at 150 g/L (corresponding to a total sugar concentration in all media of 100 g/L), a feeding time of 3 h and feeding rate of 25 mL/h achieved the highest PB and QB. The most effective results were obtained by combining a fed-batch culture with a GS system, which boosted the total PB to 23.04 g/L, PABE to 35.91 g/L and QB to 0.64 g/L·h with a butanol yield of 0.30 g/g. These values are a 14–15% improvement over the non-GS fed-batch process. The study findings demonstrate that utilizing LS as a low-cost immobilization carrier, coupled with product recovery (GS) in batch/fed-batch modes, significantly improves butanol production efficiency.

Why this record is monitored

This record has an Impact Signal of 72/100 based on recency, source, collaboration, and bibliographic signals. It prioritizes monitoring and is not a judgment of research quality.

Related topics: Biofuel production and bioconversion · Sugarcane Cultivation and Processing · Microbial Metabolic Engineering and Bioproduction