Information from the abstract
Recent reports indicate that stem cell spheres might offer enhanced therapeutic benefits by promoting cell engraftment ability, stemness, angiogenesis, and chemotaxis.Hence, we investigated whether amniotic fluid stem cells (AFSC) spheres could provide therapeutic benefits in stress urinary incontinence (SUI) due to their urethral sphincter-specific commitment.The isolated human AFSCs were characterized by flow-cytometry-based immunophenotyping and their multidifferentiation potential (osteo-, adipo-, and chondrogenic lineages).Time-dependent (＞12, 16∼20, and 48 hours) culture conditions for AFSCsphere formation were optimized.Urodynamic parameters, including leak point pressure (LPP) and intercontraction interval (ICI), were determined.hAFSCsphere with ∼150 μm diameter with minimal core necrosis at 16∼20 hours was found optimal for therapeutic application in the pudendal nerve injury-induced SUI rat model.The AFSCs demonstrated mesenchymal stem cell characteristics and multi-differentiation capabilities with retained levels of pluripotency and neural progenitor markers, SOX2 and nestin, respectively.Compared to AFSC, the AFSCsphere group showed superior LPP and ICI in rats; however, with either modest, relatively low, or unchanged levels of myogenic-lineage genes (myf5, myoD, myogenin, and desmin) between them.Bladder and external urethral sphincter histologic architecture were also improved.Notably, AFSCsphere showed the elevated levels of secetome (VEGF, IL-6, IL-8, IL-9, and MIP-1β) than AFSC, when compared to control.This could be attributed to non-myogenic pathways, such as paracrine dominance, including anti-inflammatory, angiogenic, or neurotrophic.Conclusively, our study revealed that AFSCsphere may improve urodynamics despite a significant increase in myogenic regulatory factor expression.Therefore, future studies should quantify AFSCsphere-specific properties, like stemness and cell-cell interactions, in reaching enhanced therapeutic outcomes.
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Related topics: Pelvic floor disorders treatments · Tissue Engineering and Regenerative Medicine · Urinary Bladder and Prostate Research
Thai researcher and institutional participation
Navneet Kumar Dubey · Shinawatra University
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