Papiliotrema floralis Kodchasee, Kathongthung, Senwanna, J. Kumla & N. Suwannar. 2026, sp. nov.

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Papiliotrema floralis Kodchasee, Kathongthung, Senwanna, J. Kumla & N. Suwannar. sp. nov. Fig. 3 Etymology. The specific epithet “ floralis ” refers to being associated with flowers, from which this species was isolated. Holotype. Thailand • Chiang Mai Province, Mueang District, Chang Phueak, in the Spanish needle flower (Bidens pilosa), July 2024, C. Kathongthung, P. Kodchasee, C. Senwanna, J. Kumla, and N. Suwannarach, CMUB 40151, holotype (preserved in metabolically inactive state). Ex-type living culture SDBR-CMU 888 = GMBCC 2530 = TBRC 21861. GenBank accession numbers: PZ 298078 (ITS), PZ 298097 (D 1 / D 2), PZ 309737 (rpb 1), PZ 309743 (rpb 2), PZ 309749 (tef 1 - α). Description. The culture on YMA after 5 days at 25 ° C, colonies are circular form (3.0–4.0 mm in diameter), yellowish white, smooth surface, glistening and viscous appearance, convex elevation, and entire margin. The cells are ovoid and ellipsoidal (2.40–4.31 × 3.54–6.91 μm, n = 50), occur singly, in pairs, or in short chain. Budding is multilateral. Extracellular capsule is produced (0.53–1.93 μm in thickness, n = 50). Ballistoconidia are not produced. In Dalmau plates after 2 weeks on PDA and CMA at 25 ° C, neither pseudohyphae nor true hyphae are formed. Basidiospores are not obtained for individual strains on YMA, YPDA, CMA, 5 % MEA, PDA, Fowell’s acetate agar, and Gorodkowa agar after incubation at 25 ° C for 1 month. Fermentation of glucose is negative. D - glucose, D - galactose, N - acetylglucosamine, D - xylose (weak), L - arabinose, L - rhamnose, sucrose, maltose, α, α-trehalose (weak), methyl-α- D - glucoside (weak), cellobiose, salicin (weak), melibiose, lactose, raffinose, melezitose, glycerol (weak), erythritol (weak), D - glucitol, D - mannitol, galactitol, myo - inositol, D - gluconate, and xylitol (weak) are assimilated as carbon sources, whereas L - sorbose, D - ribose, D - arabinose, inulin, soluble starch, ribitol, D - glucono- 1, 5 - lactone, D - glucuronate, D - galacturonic acid, DL - lactate, succinate, citrate, methanol, and ethanol are not assimilated. Ammonium sulfate, ethylamine hydrochloride, and L - lysine are assimilated as sole nitrogen sources, whereas potassium nitrate, sodium nitrite, and cadaverine are not assimilated. Growth occurs on media containing 50 % or 60 % glucose and on medium containing 10 % NaCl supplemented with 5 % glucose. No growth occurs on vitamin-free medium or on media containing 16 % NaCl supplemented with 5 % glucose, 0.01 % cycloheximide, or 0.1 % cycloheximide. Acid formation is weakly positive. Growth occurs at 10 ° C (slow), 15 ° C, 25 ° C, and 30 ° C but not at 37 ° C or 45 ° C. Additional strain examined. Thailand • Chiang Mai Province, Phrao District, Nam Phrae, in the oncidium flower (Oncidium sp.), July 2024, C. Kathongthung, P. Kodchasee, C. Senwanna, J. Kumla, and N. Suwannarach, living culture SDBR-CMU 892. GenBank accession numbers: PZ 298079 (ITS), PZ 298098 (D 1 / D 2), PZ 309738 (rpb 1), PZ 309744 (rpb 2), PZ 309750 (tef 1 - α). Note. Two strains, SDBR-CMU 888 and SDBR-CMU 892, representing P. floralis, formed a distinct lineage that was sister to P. anthoicola and clustered with P. rajasthanensis and P. pollinicola (Fig. 1). Data on nucleotide divergence in the ITS, D 1 / D 2, rpb 1, rpb 2, and tef 1 - α sequences are presented above (Table 2). The morphological, biochemical, and physiological characteristics distinguishing P. floralis from P. anthoicola are presented above. Papiliotrema floralis can be distinguished from P. pollinicola by its production of capsules, whereas P. pollinicola does not produce capsules (Kodchasee et al. 2026). The multilateral budding in P. floralis differs from the polar budding observed in P. rajasthanensis (Saluja and Prasad 2007). Papiliotrema floralis can be distinguished from P. pollinicola by its inability to assimilate L - sorbose, D - ribose, D - arabinose, D - glucono- 1, 5 - lactone, D - glucuronate, D - galacturonic acid, DL - lactate, succinate, citrate, and ethanol, whereas P. pollinicola can assimilate these substrates (Kodchasee et al. 2026). Additionally, P. floralis can be distinguished from P. rajasthanensis by its inability to assimilate L - sorbose, D - ribose, D - arabinose, inulin, D - glucono- 1, 5 - lactone, D - glucuronate, succinate, citrate, ethanol, and cadaverine (Saluja and Prasad 2007). Based on a polyphasic taxonomic approach, P. floralis, represented by strains SDBR-CMU 888 and SDBR-CMU 892, is described as a new species.

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Related topics: Mycorrhizal Fungi and Plant Interactions · Plant Pathogens and Fungal Diseases · Genomics and Phylogenetic Studies