Papiliotrema anthoicola Kathongthung, Kodchasee, Senwanna, J. Kumla & N. Suwannar. 2026, sp. nov.

Information from the abstract

Papiliotrema anthoicola Kathongthung, Kodchasee, Senwanna, J. Kumla & N. Suwannar. sp. nov. Fig. 2 Etymology. The specific epithet “ anthoicola ” named for inhabiting flowers, the substrate from which type of strain was first isolated. Holotype. Thailand • Chiang Mai Province, Phrao District, Nam Phrae, in the ylang-ylang flower (Cananga odorata), August 2024, C. Kathongthung, P. Kodchasee, C. Senwanna, J. Kumla, and N. Suwannarach, CMUB 40150, holotype (preserved in metabolically inactive state). Ex-type living culture SDBR-CMU 883 = GMBCC 2525 = TBRC 21859. GenBank accession numbers: PZ 298074 (ITS), PZ 298093 (D 1 / D 2), PZ 309733 (rpb 1), PZ 309739 (rpb 2), PZ 309745 (tef 1 - α). Description. The culture on YMA after 5 days at 25 ° C, colonies are circular form (2.0–3.0 mm in diameter), yellowish white, smooth surface, glistening appearance, convex elevation, and entire margin. The cells are ovoid and ellipsoidal (2.63–5.78 × 4.48–7.79 μm, n = 50), occur singly or in pairs. Budding is polar. Extracellular capsule is produced (0.47–1.63 μm in thickness, n = 50). Ballistoconidia are not produced. In Dalmau plates after 2 weeks on PDA and cornmeal agar at 25 ° C, elongated cells and chains of cells are presence on PDA, but neither pseudohyphae nor true hyphae are formed. Basidiospores are not obtained for individual strains on YMA, YPDA, CMA, 5 % MEA, PDA, Fowell’s acetate agar, and Gorodkowa agar after incubation at 25 ° C for 1 month. Fermentation of glucose is negative. D - glucose, D - galactose, N - acetylglucosamine, D - ribose (weak), D - xylose (weak), L - arabinose, L - rhamnose, sucrose, maltose, α, α-trehalose, methyl-α- D - glucoside (weak), cellobiose, salicin (weak), melibiose, lactose, raffinose, melezitose, glycerol (weak), erythritol, D - mannitol, myo - inositol, D - glucono- 1, 5 - lactone, D - gluconate, D - galacturonic acid, succinate, and xylitol (weak) are assimilated as carbon sources, whereas L - sorbose, D - arabinose, inulin, soluble starch, ribitol, D - glucitol, galactitol, D - glucuronate, DL - lactate, citrate, methanol, and ethanol are not assimilated. Ammonium sulfate, ethylamine hydrochloride, and L - lysine are assimilated as sole nitrogen sources, whereas potassium nitrate, sodium nitrite, and cadaverine are not assimilated. Growth occurs on media containing 50 % glucose (weak) or 60 % glucose (weak) and on medium containing 10 % NaCl supplemented with 5 % glucose. No growth occurs on vitamin-free medium or on media containing 16 % NaCl supplemented with 5 % glucose, 0.01 % cycloheximide, or 0.1 % cycloheximide. Acid formation is positive. Growth occurs at 10 ° C, 15 ° C, 25 ° C, 30 ° C, and 37 ° C but not at 45 ° C. Additional strains examined. Thailand • Chiang Mai Province, Mueang District, Chang Phueak, in the frangipani flower (Plumeria obtusa), August 2024, C. Kathongthung, P. Kodchasee, C. Senwanna, J. Kumla, and N. Suwannarach, living culture SDBR-CMU 893, • ibid., in the golden trumpet flower (Allamanda cathartica), July 2024, C. Kathongthung, P. Kodchasee, C. Senwanna, J. Kumla, and N. Suwannarach, living culture SDBR-CMU 895; Phrao District, Nam Phrae, in the ylang-ylang flower (Cananga odorata), August 2024, C. Kathongthung, P. Kodchasee, C. Senwanna, J. Kumla, and N. Suwannarach, living culture SDBR-CMU 894. GenBank accession numbers SDBR-CMU 893: PZ 298075 (ITS), PZ 298094 (D 1 / D 2), PZ 309734 (rpb 1), PZ 309740 (rpb 2), PZ 309746 (tef 1 - α); SDBR-CMU 894: PZ 298076 (ITS), PZ 298095 (D 1 / D 2), PZ 309735 (rpb 1), PZ 309741 (rpb 2), PZ 309747 (tef 1 - α); SDBR-CMU 895: PZ 298077 (ITS), PZ 298096 (D 1 / D 2), PZ 309736 (rpb 1), PZ 309742 (rpb 2), PZ 309748 (tef 1 - α). Note. In the phylogenetic tree, strains SDBR-CMU 883, SDBR-CMU 893, SDBR-CMU 894, and SDBR-CMU 895 of P. anthoicola formed a distinct lineage that was sister to P. floralis and clustered with P. rajasthanensis and P. pollinicola (Fig. 1). Data on nucleotide divergence in the ITS, D 1 / D 2, rpb 1, rpb 2, and tef 1 - α sequences are presented above (Table 2). Morphologically, P. anthoicola is similar to P. floralis and P. rajasthanensis in possessing extracellular capsules surrounding the cells, whereas such capsules were not observed in P. pollinicola (Saluja and Prasad 2007; Kodchasee et al. 2026). The ovoid to ellipsoidal cells of P. anthoicola can be distinguished from the oval cells of P. rajasthanensis (Saluja and Prasad 2007). The development of elongated cells in P. anthoicola on PDA after 2 weeks at 25 ° C differed from that in P. floralis, which did not form elongated cells. Physiologically, P. anthoicola differs from P. floralis in its ability to assimilate D - ribose, D - glucono- 1, 5 - lactone, D - galacturonic acid, and succinate, whereas P. floralis cannot utilize these substrates (Table 3). Moreover, P. anthoicola can grow at 37 ° C, whereas P. floralis cannot. Compared with P. pollinicola, P. anthoicola lacks the ability to assimilate L - sorbose, D - arabinose, ribitol, D - glucitol, galactitol, D - glucuronate, DL - lactate, citrate, and ethanol, which P. pollinicola can utilize (Kodchasee et al. 2026). Additionally, P. anthoicola can be distinguished from P. rajasthanensis by its ability to assimilate glycerol and grow on media containing 50 % and 60 % glucose and at 37 ° C, whereas P. rajasthanensis cannot grow under these conditions (Saluja and Prasad 2007). Consequently, P. anthoicola, represented by strains SDBR-CMU 883, SDBR-CMU 893, SDBR-CMU 894, and SDBR-CMU 895, is introduced as a novel species based on a polyphasic approach.

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Related topics: Mycorrhizal Fungi and Plant Interactions · Plant Pathogens and Fungal Diseases · Genomics and Phylogenetic Studies