Adlay (Coix lacryma-jobi L.) Extracts for Protecting Oxidative Stress and Enhancing Collagen Synthesis in Skin Fibroblast Cells (HFF-1)

Information from the abstract

Aging is a natural biological process characterized by the progressive decline of cellular functions, leading to skin alterations such as wrinkle formation and reduced regenerative capacity. Key contributing factors include oxidative stress and the loss of collagen. This study investigates the effects of Coix lacryma-jobi L. (adlay) extracts, which are rich in antioxidant compounds, on human fibroblast (HFF-1) cells. Two types of crude extracts derived from raw (CLR) and cooked (CLC) adlay seeds were prepared using 95% ethanol via ultrasonic-assisted extraction. High-performance liquid chromatography (HPLC) and Ultra-high performance liquid chromatography-Mass spectrometry (UHPLC-MS) were employed to quantify, and identify bioactive compounds, respectively. Cell viability was assessed using the MTT assay, demonstrating that adlay extracts exhibited no cytotoxicity, even at concentrations up to 1,600 µg/mL. The CLC extract significantly reduced intracellular reactive oxygen species (ROS) levels. In addition, a combination of L-ascorbic acid and adlay extracts (50 µg/mL) protected cells against H₂O₂-induced oxidative stress. Co-treatment with CLC and L-ascorbic acid enhanced collagen production in fibroblast cells. These findings suggest that cooked adlay extract has potential for further investigation as a natural source of bioactive compounds for applications related to oxidative stress mitigation and collagen synthesis.

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Related topics: Microbial Metabolism and Applications · Dyeing and Modifying Textile Fibers · Botanical Research and Applications