Aureobasidium kwanphayaoense Senwanna, Kodchasee, J. Kumla & N. Suwannar. 2026, sp. nov.

Information from the abstract

Aureobasidium kwanphayaoense Senwanna, Kodchasee, J. Kumla & N. Suwannar. sp. nov. Fig. 14 Etymology. Refers to Kwan Phayao (Phayao Lake) in Mueang Phayao District, Phayao Province, Thailand, where the flowers containing the new fungus were collected. Type. THAILAND • Phayao Province, Mueang District, isolated from chaya spinach flower (Cnidoscolus aconitifolius; Euphorbiaceae, Malpighiales), Aug 2024, P. Kodchasee, C. Senwanna, J. Kumla and N. Suwannarach, holotype = CMUB 40139 (preserved in metabolically inactive state), living culture (ex-type) = SDBR-CMU 849 = GMBCC 2506 = TBRC 21784. Cultural characteristics. After 14 days of incubation at 25 ° C in the dark, colonies on PDA reaching 28–30 mm diam., surface flat, spreading, round with radiating margin, velvety, slimy, yellowish white (2 A 2); on MEA reaching 40–48 mm diam., surface flat, spreading, round with radiating margin, velvety, orange gray (5 B 2) at center, grayish brown (7 E 3) at the margin; on OA 42–47 mm diam., surface flat, spreading with filiform, entire margin, powdery, fluffy, light gray (3 D 1). Description. Anamorph in vitro dematiaceous hyphomycetes. Vegetative hyphae 2.8–7.2 (– 9.0) μm wide, hyaline to dark brown, branched, septate, smooth, thin-walled, getting darker and thicker with age, constricted at the septa. Conidiogenous cells 10.0–11.7 × 3.0–3.8 μm (x ̄ = 11 × 3.8 μm, n = 25), undifferentiated, intercalary, rarely terminal, producing conidia percurrently from short lateral denticles, giving rise to conidia that aggregate in a mucoid mass, or differentiated on hyphae, hyaline, becoming dark brown. Conidia obovoid to ellipsoidal, elongated, or globose to subglobose, hyaline to brown, becoming dark brown with age, aseptate, occasionally with an indistinct truncated base and a hilum, guttulate, smooth-walled, (4.0 –) 5.8–11.0 (– 14.2) × 2.0–4.4 (– 7.0) μm (x ̄ = 8.4 × 3.5 μm, n = 150). Chlamydospores (6.0 –) 7.0–14.5 (– 17.6) (x ̄ = 8.9 μm, n = 25) µm wide, produced in aerial hyphae, mostly intercalary, solitary or in branched chains, globose to subglobose, dark brown, smooth-, thick-walled, septate, constricted near the septa. Endoconidia absent. Fermentation of glucose is negative. D - glucose, galactose, sorbose, N - acetyl glucosamine, ribose, xylose, L - arabinose, D - arabinose, L - rhamnose, sucrose, maltose, α - α - trehalose, methyl- α - D - glucoside, cellobiose, salicin, melibiose, lactose, raffinose, melizitose, glycerol, erythritol, ribitol, glucitol, mannitol, galactitol, myo - inositol, D - glucono- 1, 5 - lactone, D - gluconate, D - glucuronate, D - galacturonic acid, DL - lactate, succinate, citrate, ethanol, and xylitol are assimilated, but inulin, soluble starch, and methanol are not assimilated. Ammonium sulfate, ethylamine hydrochloride, L - lysine, cadaverine, and creatine are assimilated as sole nitrogen sources. Potassium nitrate and sodium nitrite are not assimilated. After 3 weeks, dark brown sediment formed in L-sorbose, salicin, erythritol. Black sediment formed in D - glucose, D - arabinose, methyl- α - D - glucoside, raffinose, glycerol, D - glucitol, myo - inositol, D - glucono- 1, 5 - lactone, D - gluconate, and ethanol. Cardinal temperatures for growth. Minimum 10 ° C, optimum in the range of 25 ° C to 30 ° C. No growth at 4 ° C and 37 ° C. Additional strains examined. THAILAND • Phayao Province, Mueang District isolated from peregrina flower (Jatropha integerrima; Euphorbiaceae, Malpighiales), Aug 2024, P. Kodchasee, C. Senwanna, J. Kumla and N. Suwannarach, living culture = SDBR-CMU 839; isolated from holy basil flower (Ocimum tenuiflorum; Lamiaceae; Lamiales), Aug 2024, P. Kodchasee, C. Senwanna, J. Kumla and N. Suwannarach, living culture = SDBR-CMU 844; isolated from purslane flower (Portulaca grandiflora; Portulacaceae, Caryophyllales), Aug 2024, P. Kodchasee, C. Senwanna, J. Kumla and N. Suwannarach, living culture = SDBR-CMU 860. Habitat and distribution. Flowers of Cnidoscolus aconitifolius, Jatropha integerrima, Ocimum tenuiflorum, and Portulaca grandiflora. Known only from northern Thailand. Notes. The multilocus phylogenetic tree (Fig. 2) showed that A. kwanphayaoense formed a distinct clade, supported by 100 % MLBS and 1.0 BIPP, and was separated from other Aureobasidium species in the A. thailandense group. Aureobasidium kwanphayaoense formed a sister clade to A. albui, A. anthicola, A. castaneae, A. florale, A. floricola, A. florigenum, A. lannaense, and A. vanuatuense. Morphologically, the colony characteristics of A. kwanphayaoense differed from those of most phylogenetically related sister taxa but were similar to those of A. albui, A. florale, and A. lannaense. However, endoconidia are absent in A. kwanphayaoense, whereas they are present in A. albui and A. florale. In addition, A. kwanphayaoense can be distinguished from these taxa based on multilocus phylogenetic analyses and nucleotide differences in the rpb 2 and tef 1 loci (Table 2). Moreover, the results of the PHI test further supported the genetic distinctiveness of A. kwanphayaoense within the A. thailandense group (Fig. 4). Furthermore, A. kwanphayaoense can be distinguished from A. anthicola, A. florale, A. floricola, A. lannaense, and A. florigenum by its characteristic pattern of sediment formation in carbon assimilation tests (Table 1). Aureobasidium kwanphayaoense can be distinguished from A. anthicola by the absence of pigmentation on salicin, D - glucono- 1, 5 - lactone, and D - gluconate, which is characteristic of A. anthicola (Table 1). In addition, A. kwanphayaoense also differs from A. floricola in its ability to assimilate methyl-α- D - glucoside. Aureobasidium albui and A. vanuatuense are clearly separated from A. kwanphayaoense by their inability to assimilate L - arabinose, L - rhamnose, salicin, glycerol, ribitol, myo - inositol, ethanol, and xylitol as carbon sources, as well as creatine as a nitrogen source (Jumbam et al. 2025). Based on the combined morphological, physiological, and molecular evidence, A. kwanphayaoense is described herein as a new species. “ – ” indicates no result due to unavailable sequence data.

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Related topics: Plant Pathogens and Fungal Diseases · Mycorrhizal Fungi and Plant Interactions · Slime Mold and Myxomycetes Research