Aureobasidium florale Senwanna, Kodchasee, J. Kumla & N. Suwannar. 2026, sp. nov.

Information from the abstract

Aureobasidium florale Senwanna, Kodchasee, J. Kumla & N. Suwannar. sp. nov. Fig. 11 Etymology. Refers to its association with flowers, the substrate from which this species was isolated. Type. THAILAND • Chiang Mai Province, Mueang District, Suthep, isolated from teak flower (Tectona grandis; Lamiaceae, Lamiales), Aug 2024, P. Kodchasee, C. Senwanna, J. Kumla and N. Suwannarach, holotype = CMUB 40136 (preserved in metabolically inactive state), living culture (ex-type) = SDBR-CMU 832 = GMBCC 2501 = TBRC 21781. Cultural characteristics. After 14 days of incubation at 25 ° C in the dark, colonies on PDA reaching 35–42 mm diam., surface flat, spreading with filiform, irregular margin, velvety, slimy, yellowish white (2 A 2); on MEA reaching 33–40 mm diam., surface flat, spreading with filiform, irregular margin, velvety, slimy, orange white (5 A 2); on OA 36–40 mm diam., surface flat, spreading with filiform, irregular margin, velvety, slimy, yellowish white (2 A 2) at center, light gray (3 D 1) at the margin. Description. Anamorph in vitro dematiaceous hyphomycetes. Vegetative hyphae (2 –) 3.8–8.6 (– 14.9) μm wide, hyaline to dark brown, getting darker and thicker with age, branched, septate, smooth. Conidiogenous cells (7.5 –) 8.6–10.5 (– 12.5) × (5.5 –) 6.2–7.6 μm (x ̄ = 11.1 × 7.8 μm, n = 20), undifferentiated, intercalary, terminal, producing conidia percurrently from indistinct, short lateral denticles, giving rise to conidia that aggregate in a mucoid mass, or differentiated on hyphae, hyaline to light brown, occasionally elongated, solitary, becoming brown to dark brown with age. Conidia (3.8 –) 5.7–10.4 (– 13.6) × (2.6 –) 4–5.6 (– 7) μm (x ̄ = 7.8 × 4.8 μm, n = 200), obovoid to ellipsoidal, elongated, or globose to subglobose, hyaline becoming melanized with age, aseptate, occasionally with an indistinct truncated base and a hilum, guttulate, smooth-walled, budding occasionally observed. Chlamydospores 6.0–9.5 (– 13.6) (x ̄ = 8.5 μm, n = 25) µm wide, produced in aerial hyphae, intercalary, solitary or in branched chains, subglobose, pigmented, dark brown, smooth-, thick-walled, septate, constricted near the septa. Endoconidia (3.7 –) 4.3–5.4 (– 6) × 2.7–3.6 (– 4.5) μm (x ̄ = 4.8 × 3.3 μm, n = 25), obovoid to ellipsoidal, hyaline, occasionally seen in intercalary hyphal cells. Fermentation of glucose is negative. D - glucose, galactose, sorbose (weak), N - acetyl glucosamine, ribose (weak), xylose, L - arabinose (weak), D - arabinose (weak), L - rhamnose, sucrose, maltose, α - α - trehalose (weak), methyl- α - D - glucoside (weak), cellobiose, salicin (weak), melibiose, lactose (weak), raffinose, melizitose, glycerol (weak), erythritol (weak), ribitol (weak), glucitol (weak), mannitol (weak), galactitol (weak), myo - inositol (weak), D - glucono- 1, 5 - lactone (weak), D - gluconate (weak), D - glucuronate (weak), D - galacturonic acid (weak), succinate (weak), citrate, ethanol (weak), and xylitol (weak) are assimilated, but inulin, soluble starch, DL - lactate, and methanol are not assimilated. Ammonium sulfate, ethylamine hydrochloride, L - lysine, cadaverine, and creatine are assimilated as sole nitrogen sources. Potassium nitrate and sodium nitrite are not assimilated. After 3 weeks, light brown sediment formed in D - glucose, D - galactose, L - sorbose, N - acetyl glucosamine, D - arabinose, L - rhamnose, sucrose, maltose, α - α - trehalose, cellobiose, salicin, melibiose, lactose, raffinose, melizitose, erythritol, ribitol, D - glucitol, D - mannitol, myo - Inositol, D - glucuronate, D - galacturonic acid, succinate, citrate, and ethanol. Black sediment formed in D - ribose, D - xylose, L - arabinose, methyl- α - D - glucoside, glycerol, galactitol, D - glucono- 1, 5 - lactone, D - gluconate, and xylitol. Cardinal temperatures for growth. Minimum 10 ° C, optimum in the range of 25 ° C to 30 ° C. No growth at 4 ° C and 37 ° C. Additional strains examined. THAILAND • Chiang Mai Province, Mueang District, Suthep, isolated from teak flower (Tectona grandis; Lamiaceae, Lamiales), Aug 2024, P. Kodchasee, C. Senwanna, J. Kumla and N. Suwannarach, living culture = SDBR-CMU 830; living culture = SDBR-CMU 831; peregrina flower (Jatropha integerrima; Euphorbiaceae, Malpighiales), Aug 2024, P. Kodchasee, C. Senwanna, J. Kumla and N. Suwannarach, living culture = SDBR-CMU 840; SDBR-CMU 850; Chang Phueak, isolated from daisy flower (Thymophylla tenuiloba; Asteraceae, Asterales), Jul 2024, P. Kodchasee, C. Senwanna, J. Kumla and N. Suwannarach, living culture = SDBR-CMU 862. Habitat and distribution. Flowers of Jatropha integerrima, Tectona grandis, and Thymophylla tenuiloba. Known only from northern Thailand. Notes. The multilocus phylogenetic tree showed that A. florale SDBR-CMU 830, SDBR-CMU 831, SDBR-CMU 832 (ex-type), SDBR-CMU 840, SDBR-CMU 850, and SDBR-CMU 862 formed a well-supported monophyletic lineage (99 % MLBS and 0.98 BIPP) that was sister to A. albui, A. castaneae, A. floricola, and A. lannaense (Fig. 2). Additionally, the PHI test results (Fig. 4) indicated significant genetic differentiation, supporting the recognition of A. florale as a distinct species from A. albui, A. castaneae, A. floricola, and A. lannaense. Morphologically, colonies of A. florale on MEA are orange white, clearly differing from the grayish-brown colonies of A. albui, the olive-brown colonies of A. castaneae, the olive-brown to black colonies with a yellowish-white margin of A. floricola, and the yellowish-white and dark-gray to black colonies of A. lannaense. Although the micromorphology of A. florale is similar to that of A. albui, A. castaneae, A. floricola, and A. lannaense, these taxa can be distinguished based on multilocus phylogenetic analyses. Moreover, A. florale differs from A. lannaense by the presence of endoconidia. Furthermore, A. florale can be distinguished from A. floricola and A. lannaense by its inability to assimilate DL - lactate (Table 1). It also differs from A. floricola in its ability to assimilate methyl-α- D - glucoside. In addition, A. florale differs from A. albui in its ability to assimilate D - arabinose, L - rhamnose, salicin, glycerol, erythritol, ribitol, myo - inositol, ethanol, xylitol, and creatine (Jumbam et al. 2025). Moreover, A. florale did not produce pigmentation when grown in L - sorbose, erythritol, D - mannitol, or D - glucono- 1, 5 - lactone, whereas A. albui produced pigmentation (Jumbam et al. 2025). Hence, A. florale is introduced as a new species based on both morphological characteristics and molecular data.

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Related topics: Plant Pathogens and Fungal Diseases · Biological and pharmacological studies of plants · Genomics and Phylogenetic Studies