Aureobasidium anthicola Senwanna, Kodchasee, J. Kumla & N. Suwannar. 2026, sp. nov.

Information from the abstract

Aureobasidium anthicola Senwanna, Kodchasee, J. Kumla & N. Suwannar. sp. nov. Fig. 6 Etymology. Refers to “ inhabiting flowers, ” the substrate from which this species was first isolated. Type. THAILAND • Phayao Province, Mueang District, isolated from bridal bouquet flower (Plumeria pudica; Apocynaceae, Gentianales), Aug 2024, P. Kodchasee, C. Senwanna, J. Kumla and N. Suwannarach, holotype = CMUB 40138 (preserved in metabolically inactive state), living culture (ex-type) = SDBR-CMU 851 = GMBCC 2507. Cultural characteristics. After 14 days of incubation at 25 ° C in the dark, colonies on PDA reaching 35–43 mm diam., surface flat, spreading, round with radiating margin, velvety, concentric, light gray (3 D 1) at center, pale orange (6 A 3), olive brown (4 E 5); on MEA reaching 30–37 mm diam., surface flat, spreading, round with radiating margin, velvety, brownish gray (4 E 2) to black, with olive brown at the margin; on OA 36–40 mm diam., surface flat, spreading with filiform, with entire margin, wrinkled, light gray (3 D 1) at center, pale orange (6 A 3) mixed with olive brown (4 E 5). Description. Anamorph in vitro dematiaceous hyphomycetes. Vegetative hyphae (1.9 –) 2.9–7.7 (– 9) μm wide, hyaline to dark brown, branched, septate, smooth, thin-walled, becoming light brown to brown or golden brown with age, constricted at the septa. Conidiogenous cells (7.0 –) 9.1–14.0 (– 16.0) × 4.0–6.0 (– 8.9) μm (x ̄ = 11.4 × 6.2 μm, n = 25), undifferentiated, intercalary, terminal, producing conidia percurrently from indistinct, short lateral denticles, giving rise to conidia that aggregate in a mucoid mass, or differentiated on hyphae, hyaline to dark brown in age, occasionally elongated, solitary. Conidia (4.2 –) 6.3–10.5 (– 13.0) × (2.5 –) 3.4–6.0 (– 8.0) μm (x ̄ = 8.3 × 4.2 μm, n = 200), obovoid to ellipsoidal, hyaline to light brown, aseptate, guttulate, smooth-walled, becoming light brown to brown with ages. Chlamydospores (5.7 –) 7.2–10.4 (– 12.3) μm wide (x ̄ = 8.7 μm, n = 45), produced in aerial hyphae, mostly intercalary, solitary or in branched chains, subglobose to ellipsoidal, pigmented, light to dark brown, smooth-, thick-walled, septate, constricted near the septa. Endoconidia (2.7 –) 3.6–6.0 (– 6.6) × 2.2–3.1 (– 3.7) μm (x ̄ = 4.8 × 2.8 μm, n = 40), ellipsoidal, hyaline, occasionally seen in intercalary hyphal cells. Fermentation of glucose is negative. D - glucose, galactose, sorbose, N - acetyl glucosamine, ribose, xylose, L - arabinose, D - arabinose, L - rhamnose, sucrose, maltose, α - α - trehalose, methyl- α - D - glucoside, cellobiose, salicin, melibiose, lactose, raffinose, melizitose, glycerol, erythritol, ribitol, glucitol, mannitol, galactitol, myo - inositol, D - glucono- 1, 5 - lactone, D - gluconate, D - glucuronate, D - galacturonic acid, DL - lactate, succinate, citrate, ethanol, and xylitol are assimilated, but inulin, soluble starch, and methanol are not assimilated. Ammonium sulfate, ethylamine hydrochloride, L - lysine, cadaverine, and creatine are assimilated as sole nitrogen sources. Potassium nitrate and sodium nitrite are not assimilated. After 3 weeks, light brown pigmentation was observed in salicin, D - glucono- 1, 5 - lactone, and D- glucuronate, while dark brown sediment formed in D - galactose, D - arabinose, sucrose, methyl- α - D - glucoside, raffinose, and D - gluconate. Cardinal temperatures for growth. Minimum 10 ° C, optimum in the range of 25 ° C to 30 ° C. No growth at 4 ° C and 37 ° C. Additional strains examined. THAILAND • Chiang Mai Province, Mueang District, Suthep, isolated from Fukien tea flower (Ehretia microphylla; Boraginaceae, Boraginales), Jul 2024, P. Kodchasee, C. Senwanna, J. Kumla and N. Suwannarach, living culture = SDBR-CMU 821. Habitat and distribution. Flowers of Ehretia microphylla and Plumeria pudica. Known only from northern Thailand. Notes. Based on the multilocus phylogenetic analyses, A. anthicola SDBR-CMU 821 and SDBR-CMU 851 (ex-type) formed a well-supported monophyletic lineage (100 % MLBS and 0.99 BIPP) that was sister to A. florigenum (Fig. 2). The rpb 2 and tef 1 sequences of A. anthicola differed from those of A. florigenum by 1.74 % (17 / 977 bp, including gaps) and 1.88 % (5 / 266 bp, including gaps), respectively. Additionally, the PHI test confirmed the genetic distinctiveness of A. anthicola and A. florigenum (Fig. 4). Morphologically, colonies of A. anthicola on OA differed from those of A. florigenum by having a pale orange mixed with olive-brown color, whereas A. florigenum produced yellowish-white colonies mixed with light gray and with a brownish-gray margin. Aureobasidium anthicola produced endoconidia, whereas A. florigenum did not. Based on physiological characteristics, A. anthicola differed from A. florigenum by producing light brown pigment when grown in salicin, D - glucono- 1, 5 - lactone, and D - glucuronate, which was not produced by A. florigenum. In addition, differences in sediment formation were observed during carbon assimilation (Table 1). Therefore, A. anthicola is introduced as a new species in the A. thailandense group.

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Related topics: Plant Pathogens and Fungal Diseases · Biological and pharmacological studies of plants · Genomics and Phylogenetic Studies