Information from the abstract
cells were transduced with a STAT6-responsive luciferase reporter using a lentiviral vector, followed by optimization of puromycin selection and multiplicity of infection, and monoclonal isolation by limiting dilution. A stable clone with strong and reproducible induction across serial passages was selected. Reporter responsiveness was validated by IL-4/IL-13 stimulation, and STAT6 dependence was confirmed using selective STAT6, STAT5, and STAT3 inhibitors. Assay performance was quantified by Z'-factor analysis, which indicated reproducible signal separation. Furthermore, the assay was applied to individual air-pollution constituents, and benzo[b]fluoranthene and particulate matter significantly increased STAT6 reporter activity. This method provides a scalable approach for measuring STAT6 activity in airway epithelial cells and for prioritizing environmental toxicants that modulate allergic airway signaling.
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Related topics: Asthma and respiratory diseases · Psoriasis: Treatment and Pathogenesis · Allergic Rhinitis and Sensitization
Thai researcher and institutional participation
Aekkacha Moonwiriyakit · Phattarin Pothipan · Dhanpisit Toyingsirikul · Pawit Santiwong · Sirapop Lusawat · Ramathibodi Hospital · Mahidol University
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