A Nigerian laboratory study compared aqueous and n-hexane Vernonia amygdalina leaf extracts, using phytochemical screening and FTIR before agar-well-diffusion testing against selected multidrug-resistant ESKAPE isolates. Extraction yield and chemical fingerprints varied by solvent, with in vitro inhibition signals.
Key findings
- n-Hexane yield was 28.81% versus 9.87% for water (p
Why this matters globally
New antimicrobial leads matter for AMR, but a credible path requires compound isolation, structure identification, potency and selectivity, mechanism and resistance testing, and formulation work.
Thai researcher contribution
Chiang Mai University- and Chulalongkorn University-affiliated authors joined Nigerian collaborators in phytochemistry, FTIR, and microbiology. Plant material and isolates came from Nigeria, not Thailand.
Limitations to consider
Agar diffusion depends on compound mobility and does not directly provide MIC/MBC. FTIR functional groups do not identify specific molecules. Replication details, cytotoxicity, stability, and in vivo evidence are missing, and p=0.066 is not a non-inferiority test.
Verify the original sources
Acta Microbiologica HellenicaRead the original article↗DOI: 10.3390/amh71030019