Three antibody LFAs targeting Hcp1, GroEL and whole-cell lysate used protein-G colloidal gold and were tested on 31 positive and 51 negative Mongolian equine sera plus 60 other-infection sera from Japan.
Key findings
- Sensitivity/specificity were 100/100% for Hcp1, 100/98.0% for WCL and 100/90.2% for GroEL. No cross-reactivity occurred in the Japanese panel and all assays remained functional for at least 16 weeks at 37°C.
Why this matters globally
Glanders is a zoonotic and trade-relevant disease. Low-resource field tests could be valuable, but case-control performance can exceed that in low-prevalence routine settings.
Thai researcher contribution
Bibliographic records link authors to a Department of Disease Control in Thailand; the full article should be consulted for precise institutional attribution.
Limitations to consider
Only 31 positives were tested from one setting. Prospective field workflow, lot variation and predictive values were not assessed, and perfect estimates have wide confidence intervals.